Labchip® low pi charge variant analysis
WebThe PerkinElmer LabChip charge variant analysis chips allow you to screen and analyze the charge variant profiles of protein samples fast and easily on LabChip GXII Touch™ HT … WebThe LabChip ® GXII Touch ™ protein characterization system offers rapid peak quantification and quality control throughout the protein purification workflow. Analysis can be performed in as few as forty seconds per sample that deliver comparable data to traditional capillary electrophoresis with as much as a 70X increase in throughput.
Labchip® low pi charge variant analysis
Did you know?
WebPN CLS154075 Rev. A Page 1 of 3 Low pI Charge Variant Assay Quick Guide LabChip® GXII Touch Note: We highly recommend that first-time users read the full Low pI Charge Variant Assay User Guide before proceeding. Critical: Allow the chip and all refrigerated reagents to equilibrate to room temperature (20 - 25°C) for at least 30 minutes before use. ... http://www.caliperls.com/assets/032/9594.pdf
WebCapillary isoelectric focusing (cIEF) is a high-resolution analytical technique that allows the separation of protein/peptide mixtures, protein glycoforms and other charge variants, based on their isoelectric point (pI). cIEF is based on the principle of … WebThe Protein Charge Variant Assay is for use with the LabChip GXII Touch instrument. LabChip GXII Touch instruments are for research use only and not for use in diagnostic procedures. Preparation Procedures 6 PN CLS140162 Rev. C Protein Charge Variant Assay User Guide PerkinElmer, Inc. Preparation Procedures Additional Items Required
Webprofile by microchip CE-SDS, and profiling of N-glycans with analysis times of less than 60 seconds per sample. More recently we have developed a high throughput microchip-CZE method for determination of antibody charge heterogeneity. In this method, antibodies in the pI range of 7.5 to 9 are fluorescently labeled in a 10 minute WebMar 24, 2024 · This chromatogram shows 6% of acidic variants, 24% of basic forms due in part to the presence of C-terminal lysines and a main peak eluting at 16.1 min, corresponding to the Fc/2 dimer without C-terminal lysines and accounting for 70% of the Fc/2-related total UV area.
WebA Low pI Charge Variant Assay User Guide PerkinElmer, Inc. Preparing the Chip 1Allow the chip to equilibrate to room temperature (20 - 25°C) for at least 30 minutes before use. …
Webcharge variant ratios induced during manufacture, storage and transport, can potentially shift the pI of the protein, leading to detrimental effects on drug activity1. Analytical methods that can monitor charge variant ratios during manufacture and lot release are therefore essential to ensure drug safety and efficacy. crystal store henderson nvWebIn monoclonal antibodies (mAb) quality control, cIEF is used for the detection of charge variants including lysine clipped and deaminated impurities of a protein which are process driven modifications of the parent mAb and has been used in quality control of mAb produced by biopharmaceutical companies for over 10 years (1). dynamic afs headlightsWebThe LabChip ® Extended Range N-Linked Glycan assay is ideal for rapid screening of N-linked glycan profiles by combining: Plate based reagents in 96-well format for convenience and ease of use in automated liquid handlers such as the JANUS® G3 workstation from PerkinElmer. Rapid enzymatic deglycosylation with PNGase F in 96 well format. dynamic ag solutions ogden iaWebThe PerkinElmer LabChip charge variant analysis assays allow you to efficiently analyze the charge variant profiles of your protein samples. crystal store huntington wvWebVersatile instrument for protein analysis and quantitation Multiple assays available for quantification, molecular weight sizing, purity, charge variants and glycan analysis. With our pre-verified assays, there is no (or little) method development needed. crystal store hendersonville ncWebThe LabChip ® GXII Touch ™ protein characterization system provides the complete solution for reproducible quantitation, molecular weight sizing and percent purity analysis of protein samples. Leveraging microfluidic electrophoretic separation technology, the LabChip ® GXII Touch ™ system enables rapid characterization with minimal sample preparation … dynamic agriculture years 7-10 4th editionWebNov 1, 2011 · Analyzing charge variants of therapeutic proteins is critical for characterizing and monitoring quality attributes of antibodies. Charge variants include deamidation, formation of N-terminal pyroglutamate, aggregation, isomerization, sialylated glycans, antibody fragmentation, and glycation at the lysine residues. In some cases, such … crystal store herndon va