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Hoechst 33342 molecular probes

Nettet28. feb. 2024 · Hoechst 33342 staining also showed obvious morphological characteristics of apoptosis in T24 cells incubated with Au–Ag@PDA NPs at 3 and 24 hours after laser irradiation. In particular, the apoptotic cells stained with Hoechst 33342 presented brighter blue fluorescence due to karyopyknosis and chromatin condensation in the … Nettet(Molecular Probes: C-1430). Hoechst 33342, a bisbenzimide dye, is a cell membrane permeant, minor groove-binding DNA stain that fluoresce bright blue upon binding to DNA. The dye is quite soluble in water and relatively non-toxic (Molecular Probes: H-1399, H-3570). Hoechst 33342 stains both, live and dead cells. Material and Methods Cell Culture

Protocol to Count Cell Number of Preimplantation Embryos using …

Nettet4. nov. 2016 · The influence of ABC transporters on the fluorescence of probes in SP and MP cells. FACS plots and mean fluorescent intensity (MFI) of sorted C6 SP (Hoechst 33342-effluxing) and MP (Hoechst 33342-retaining) cells after staining with CellROX (a, b), MTG (c, d) and JC-1 (e-g) in the presence or absence of verapamil.Fluorescence of … NettetHoechst 33342 Binding. Hoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell … lvw193 https://editofficial.com

Invitrogen™ Höchst 33342, Trihydrochlorid, Trihydrat - Fisher Sci

NettetHoechst 33342, trihydrochloride, trihydrate, FluoroPure™ grade (MW 615.99) (H21492) 100 mg NA ≤25°C Protect from light • • When stored as directed, product is stable for at least 12 months. NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue™ Live ReadyProbes™ Reagent we … NettetHoechst 33342 is a bisbenzimidazole dye with high specificity for binding to double-stranded DNA (preferentially binds to A-T base pairs). This dye is very useful to label double-stranded DNA and thus to visualize nuclei. Hoechst 33342 can be excited at ~355 nm by a UV light source (eg, UV laser beam or a mercury arc-lamp). king size pillow top mattress prices

Hoechst 33342 (5117) Bio-Techne

Category:Bidirectional transport of rhodamine 123 and Hoechst 33342 ...

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Hoechst 33342 molecular probes

Hoechst 33342 solution, 1 mg/ml in water - CAS-Number 23491 …

http://www.cogershop.com/hoechst-33342.htm NettetHoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342 exhibits a 10 fold greater cell …

Hoechst 33342 molecular probes

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NettetHöchst 33342 ist eine beliebte zelldurchlässige nukleäre Gegenfärbung mit einer blauen Fluoreszenz bei Anbindung an dsDNA. Dieses Färbemittel wird häufig zur … NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed …

NettetThis bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Hoechst 34580 has … Nettet1. jan. 2009 · Hoechst 33342 is the fluorophore used routinely to measure DNA in X- and Y-chromosome-bearing mammalian sperm so they can be separated by flow sorting. A difference of <3% in DNA mass can be detected. This synthetic dye consists of two adjacent benzimidazole rings with N-methyl-piperazine and phenolic groups at the ends.

NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed … NettetDAPI has higher photostability than Hoechst 33342. Other DNA-binding dyes can also be used including propidium iodide and ethidium bromide. Staining with propidium iodide is a little more complicated because the dye also stains RNA and an RNAse step is often added. The Molecular Probes website has much useful information on the properties …

NettetHoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342 exhibits a 10 fold greater cell-permeability than H 33258. Cells can integrate BrdU in newly synthesized DNA as a substitute for thymidine.

Nettet(B) Co-staining of viscosity-sensitive probes (DSP-16 and DSP-18), Dil (a commercial plasma membrane probe) with Hoechst 33342 (a commercial nuclei probe) … king size pillow top fitted sheetsNettet20. feb. 2015 · This chapter lists the CAS registry number, chemical structure, chemical abstract (CA) index name, other chemical names, Merck Index number, chemical/dye … lvw18/ledNettetHoechst 33342, trihydrochloride, trihydrate, FluoroPure™ grade (MW 615.99) (H21492) 100 mg NA ≤25°C Protect from ... Molecular Probes, Inc. Further information on Molecular Probes products, including product bibliographies, is available from your local distributor or directly lvw192NettetHoechst 33342 contains additional ethyl group. This group renders Hoechst 33342 a bit more hydrophobic than Hoechst 33258. Both dyes have the absorption band at 350 nm and the emission band in the “blue” region. They are non-intercalating fluorescent probes for non-covalent labeling of DNA. lvw-224tNettetHoechst 33342 est un contre-colorant nucléaire populaire, perméant aux cellules, qui émet une fluorescence bleue en cas de liaison à l’ADNdb. Ce colorant est souvent utilisé … lvw222NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed … lvw223NettetBiotium offers both Hoechst 33342 and Hoechst 33258, ... Note: DAPI or Hoechst can be combined with other fluorescent probes. ... Molecular formula: C 27 H 31 Cl 3 N 6 O; CAS number: 23491-52-3; Recommended staining concentration: 1 ug/mL; Hoechst 33342, trihydrochloride trihydrate. lvw18/led/hdrp