Nettet28. feb. 2024 · Hoechst 33342 staining also showed obvious morphological characteristics of apoptosis in T24 cells incubated with Au–Ag@PDA NPs at 3 and 24 hours after laser irradiation. In particular, the apoptotic cells stained with Hoechst 33342 presented brighter blue fluorescence due to karyopyknosis and chromatin condensation in the … Nettet(Molecular Probes: C-1430). Hoechst 33342, a bisbenzimide dye, is a cell membrane permeant, minor groove-binding DNA stain that fluoresce bright blue upon binding to DNA. The dye is quite soluble in water and relatively non-toxic (Molecular Probes: H-1399, H-3570). Hoechst 33342 stains both, live and dead cells. Material and Methods Cell Culture
Protocol to Count Cell Number of Preimplantation Embryos using …
Nettet4. nov. 2016 · The influence of ABC transporters on the fluorescence of probes in SP and MP cells. FACS plots and mean fluorescent intensity (MFI) of sorted C6 SP (Hoechst 33342-effluxing) and MP (Hoechst 33342-retaining) cells after staining with CellROX (a, b), MTG (c, d) and JC-1 (e-g) in the presence or absence of verapamil.Fluorescence of … NettetHoechst 33342 Binding. Hoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell … lvw193
Invitrogen™ Höchst 33342, Trihydrochlorid, Trihydrat - Fisher Sci
NettetHoechst 33342, trihydrochloride, trihydrate, FluoroPure™ grade (MW 615.99) (H21492) 100 mg NA ≤25°C Protect from light • • When stored as directed, product is stable for at least 12 months. NettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue™ Live ReadyProbes™ Reagent we … NettetHoechst 33342 is a bisbenzimidazole dye with high specificity for binding to double-stranded DNA (preferentially binds to A-T base pairs). This dye is very useful to label double-stranded DNA and thus to visualize nuclei. Hoechst 33342 can be excited at ~355 nm by a UV light source (eg, UV laser beam or a mercury arc-lamp). king size pillow top mattress prices