WebBiosafety. Preventing Aerosol Production. Aerosols are liquid and solid particles suspended in the air. An aerosol with a diameter of 5 microns or less can remain airborne for a long period of time, spread wide distances, and is easily inhaled. Particles with a diameter larger than 5 microns tend to settle rapidly and can contaminate skin ... Web1. Place alternative flaming device in the biosafety cabinet and heat according to manufacturer’s directions. 2. Sterilize your wire inoculating loop by passing it at an angle …
Fact Sheet: Flame Sterilization on the Open Bench PennEHRS
WebThe inoculating loop/needle is sterilized to kill any contaminates it might have come in contact with in the air or culture. What is the purpose of subculturing? Subculturing keeps cells and microorganisms alive by transferring them from a previous growth culture to a fresh growth medium that allows for increased growth and multiplication. WebMay 18, 2024 · 3. With an inoculation loop or needle, pick up a small amount of bacteria. Mix it well with the saline and spread the mixture over a wider area of the slide. Be careful not to have the two smears run into each other. 4. Air dry the bacterial specimen on the slide (slide warmers may also be used). 5. holiday inn borough green
Lab 9- Aseptic Technique Flashcards Quizlet
WebJun 3, 2024 · The loop with sample is placed in the clear or blue part of the flame and the resulting color is observed. Wooden Splint or Cotton Swab Method Wooden splints or cotton swabs offer an inexpensive alternative to wire loops. To use wooden splints, soak them overnight in distilled water. WebInsert the loop into the broth without touching the sides of the tube. Twirl the loop like a swizzle stick to dislodge the bacteria. Touch the loop to the side of the tube by the mouth to remove excess fluid. Use the incinerator to sterilize the loop. Flame the mouth of the tube with the Bunsen burner and replace the cap. WebA lab coat and gloves must be worn for all experiments. True. Flaming loop or a needle until it is red hot will sterilize the wire. True. A flamed loop must be cooled for approximately 10 seconds before it is used. True. Results are compromised if a sizzling sound is heard after a flamed lop is placed on the surface of the agar. holiday inn bordentown new jersey