WebPrimer design tips. In general, a length of 18–30 nucleotides for primers is good. Try to make the melting temperature (T m) of the primers between 65°C and 75°C, and within 5°C of each other.; If the T m of your primer is very low, try to find a sequence with more GC content, or extend the length of the primer a little.; Aim for the GC content to be between … WebMaha M. Abdel-Kader, M.D.Board Certified Psychiatrist. Dr. Abdel-Kader obtained her medical degree from Cairo University, Egypt in 1994. After relocating to the United …
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WebFAM is a commonly used fluorescent dye attachment for oligonucleotides and is compatible with a variety of fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. FAM can be attached to 5' or 3' end of oligos. WebYou will anneal equal amounts of the top and bottom strand oligos to generate the ds oligos. We generally perform the annealing reaction at a final single-stranded oligo concentration of 50 µM. Annealing at concentrations lower than 50 µM can significantly reduce the efficiency. Note that the annealing step is not WebDescription. Flame resistant concealed snap-front lab coat with exterior pockets. This flame resistant lab coat is designed for functionality comfort and safety with a CAT 2 arc rating and NFPA™ 2112 compliance. It comes equipped with concealed snap front closures, adjustable straps, two chest pockets, and two front hip pockets. north and south american desserts